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mouse against dcc icd  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology mouse against dcc icd
    A Western blot of HEK293T cell lysates transfected with Dcc-pHluorin (Dcc-pH) and JamC-HALO. Immunoprecipitation was performed using GFP or negative control IgG antibodies. Blots were probed for Dcc and HALO-tag, with the yellow arrowhead indicating the expected JamC-HALO band size. B Schematic depicting interactions between Dcc, JamC, and the polarity protein Pard3. Pard3 recruits JamC to the membrane through its PDZ1 domain, which binds the Class 2 PDZ motif at JamC’s C-terminus. JamC interacts with Dcc’s extracellular domain, while Pard3’s PDZ3 the PDZ 3 domain is predicted to interact with a Class 1 PDZ binding motif (X-S/T-X-ϕ COOH ) , , present on Dcc intracellular domain. C , D Airyscan confocal imaging of CGNs nucleofected with Dcc-pHluorin (cyan), JamC-SNAP (yellow), and Halo-Pard3 (magenta). Phluorin and the SNAP dye used here are both pH sensitive highlighting membrane-bound proteins. C Single focal plane showing overlap of Dcc with JamC and Pard3 at the proximal dilation of a CGN (white arrowheads). D Maximum projection of two CGNs forming an adhesion, showing Dcc clustering at the adhesion site before and after Ntn1 addition (200 ng/L). Dcc co-localized with JamC/Pard3 at the adhesion (white arrowhead) and accumulated at the adhesion periphery (hollow arrowhead). Five minutes after the addition of Ntn1 at 200 ng/L, the number of bright Dcc clusters (blue arrowhead) at the membrane surface increased and some newly formed clusters were recruited to the periphery of the JamC/Pard3/Dcc-positive adhesion (white arrowhead). Proximity Labelling Assay (PLA) using Duolink™ fluorescence protocol on fixed dissociated granule neurons plated on laminin and cultured for 24 h, using 2 pairs of primary antibodies: Rabbit against Dcc extracellular domain (ECD) and a Goat against JamC ECD ( E ), and Mouse against Dcc <t>intracellular</t> <t>domain</t> <t>(ICD)</t> and a Rabbit against Pard3 ( F ). Duolink™ staining with no primary, only one or both primaries were compared. Bar graphs represent the ratio of PLA staining intensity (Gray) against Dapi (Cyan) intensity normalized to the negative control without primary antibody (E: n = 4, F: n = 4), replicated 4 (E) and 3 (F) times with similar results. Scale bars: (C, D) 5 µm, (E-F) 10 µm. Error bars represent SEM. See Source Data File.
    Mouse Against Dcc Icd, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse against dcc icd/product/Santa Cruz Biotechnology
    Average 93 stars, based on 66 article reviews
    mouse against dcc icd - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit"

    Article Title: Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit

    Journal: Nature Communications

    doi: 10.1038/s41467-024-55400-w

    A Western blot of HEK293T cell lysates transfected with Dcc-pHluorin (Dcc-pH) and JamC-HALO. Immunoprecipitation was performed using GFP or negative control IgG antibodies. Blots were probed for Dcc and HALO-tag, with the yellow arrowhead indicating the expected JamC-HALO band size. B Schematic depicting interactions between Dcc, JamC, and the polarity protein Pard3. Pard3 recruits JamC to the membrane through its PDZ1 domain, which binds the Class 2 PDZ motif at JamC’s C-terminus. JamC interacts with Dcc’s extracellular domain, while Pard3’s PDZ3 the PDZ 3 domain is predicted to interact with a Class 1 PDZ binding motif (X-S/T-X-ϕ COOH ) , , present on Dcc intracellular domain. C , D Airyscan confocal imaging of CGNs nucleofected with Dcc-pHluorin (cyan), JamC-SNAP (yellow), and Halo-Pard3 (magenta). Phluorin and the SNAP dye used here are both pH sensitive highlighting membrane-bound proteins. C Single focal plane showing overlap of Dcc with JamC and Pard3 at the proximal dilation of a CGN (white arrowheads). D Maximum projection of two CGNs forming an adhesion, showing Dcc clustering at the adhesion site before and after Ntn1 addition (200 ng/L). Dcc co-localized with JamC/Pard3 at the adhesion (white arrowhead) and accumulated at the adhesion periphery (hollow arrowhead). Five minutes after the addition of Ntn1 at 200 ng/L, the number of bright Dcc clusters (blue arrowhead) at the membrane surface increased and some newly formed clusters were recruited to the periphery of the JamC/Pard3/Dcc-positive adhesion (white arrowhead). Proximity Labelling Assay (PLA) using Duolink™ fluorescence protocol on fixed dissociated granule neurons plated on laminin and cultured for 24 h, using 2 pairs of primary antibodies: Rabbit against Dcc extracellular domain (ECD) and a Goat against JamC ECD ( E ), and Mouse against Dcc intracellular domain (ICD) and a Rabbit against Pard3 ( F ). Duolink™ staining with no primary, only one or both primaries were compared. Bar graphs represent the ratio of PLA staining intensity (Gray) against Dapi (Cyan) intensity normalized to the negative control without primary antibody (E: n = 4, F: n = 4), replicated 4 (E) and 3 (F) times with similar results. Scale bars: (C, D) 5 µm, (E-F) 10 µm. Error bars represent SEM. See Source Data File.
    Figure Legend Snippet: A Western blot of HEK293T cell lysates transfected with Dcc-pHluorin (Dcc-pH) and JamC-HALO. Immunoprecipitation was performed using GFP or negative control IgG antibodies. Blots were probed for Dcc and HALO-tag, with the yellow arrowhead indicating the expected JamC-HALO band size. B Schematic depicting interactions between Dcc, JamC, and the polarity protein Pard3. Pard3 recruits JamC to the membrane through its PDZ1 domain, which binds the Class 2 PDZ motif at JamC’s C-terminus. JamC interacts with Dcc’s extracellular domain, while Pard3’s PDZ3 the PDZ 3 domain is predicted to interact with a Class 1 PDZ binding motif (X-S/T-X-ϕ COOH ) , , present on Dcc intracellular domain. C , D Airyscan confocal imaging of CGNs nucleofected with Dcc-pHluorin (cyan), JamC-SNAP (yellow), and Halo-Pard3 (magenta). Phluorin and the SNAP dye used here are both pH sensitive highlighting membrane-bound proteins. C Single focal plane showing overlap of Dcc with JamC and Pard3 at the proximal dilation of a CGN (white arrowheads). D Maximum projection of two CGNs forming an adhesion, showing Dcc clustering at the adhesion site before and after Ntn1 addition (200 ng/L). Dcc co-localized with JamC/Pard3 at the adhesion (white arrowhead) and accumulated at the adhesion periphery (hollow arrowhead). Five minutes after the addition of Ntn1 at 200 ng/L, the number of bright Dcc clusters (blue arrowhead) at the membrane surface increased and some newly formed clusters were recruited to the periphery of the JamC/Pard3/Dcc-positive adhesion (white arrowhead). Proximity Labelling Assay (PLA) using Duolink™ fluorescence protocol on fixed dissociated granule neurons plated on laminin and cultured for 24 h, using 2 pairs of primary antibodies: Rabbit against Dcc extracellular domain (ECD) and a Goat against JamC ECD ( E ), and Mouse against Dcc intracellular domain (ICD) and a Rabbit against Pard3 ( F ). Duolink™ staining with no primary, only one or both primaries were compared. Bar graphs represent the ratio of PLA staining intensity (Gray) against Dapi (Cyan) intensity normalized to the negative control without primary antibody (E: n = 4, F: n = 4), replicated 4 (E) and 3 (F) times with similar results. Scale bars: (C, D) 5 µm, (E-F) 10 µm. Error bars represent SEM. See Source Data File.

    Techniques Used: Western Blot, Transfection, Immunoprecipitation, Negative Control, Membrane, Binding Assay, Imaging, Fluorescence, Cell Culture, Staining



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    mouse against dcc icd  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology mouse against dcc icd
    A Western blot of HEK293T cell lysates transfected with Dcc-pHluorin (Dcc-pH) and JamC-HALO. Immunoprecipitation was performed using GFP or negative control IgG antibodies. Blots were probed for Dcc and HALO-tag, with the yellow arrowhead indicating the expected JamC-HALO band size. B Schematic depicting interactions between Dcc, JamC, and the polarity protein Pard3. Pard3 recruits JamC to the membrane through its PDZ1 domain, which binds the Class 2 PDZ motif at JamC’s C-terminus. JamC interacts with Dcc’s extracellular domain, while Pard3’s PDZ3 the PDZ 3 domain is predicted to interact with a Class 1 PDZ binding motif (X-S/T-X-ϕ COOH ) , , present on Dcc intracellular domain. C , D Airyscan confocal imaging of CGNs nucleofected with Dcc-pHluorin (cyan), JamC-SNAP (yellow), and Halo-Pard3 (magenta). Phluorin and the SNAP dye used here are both pH sensitive highlighting membrane-bound proteins. C Single focal plane showing overlap of Dcc with JamC and Pard3 at the proximal dilation of a CGN (white arrowheads). D Maximum projection of two CGNs forming an adhesion, showing Dcc clustering at the adhesion site before and after Ntn1 addition (200 ng/L). Dcc co-localized with JamC/Pard3 at the adhesion (white arrowhead) and accumulated at the adhesion periphery (hollow arrowhead). Five minutes after the addition of Ntn1 at 200 ng/L, the number of bright Dcc clusters (blue arrowhead) at the membrane surface increased and some newly formed clusters were recruited to the periphery of the JamC/Pard3/Dcc-positive adhesion (white arrowhead). Proximity Labelling Assay (PLA) using Duolink™ fluorescence protocol on fixed dissociated granule neurons plated on laminin and cultured for 24 h, using 2 pairs of primary antibodies: Rabbit against Dcc extracellular domain (ECD) and a Goat against JamC ECD ( E ), and Mouse against Dcc <t>intracellular</t> <t>domain</t> <t>(ICD)</t> and a Rabbit against Pard3 ( F ). Duolink™ staining with no primary, only one or both primaries were compared. Bar graphs represent the ratio of PLA staining intensity (Gray) against Dapi (Cyan) intensity normalized to the negative control without primary antibody (E: n = 4, F: n = 4), replicated 4 (E) and 3 (F) times with similar results. Scale bars: (C, D) 5 µm, (E-F) 10 µm. Error bars represent SEM. See Source Data File.
    Mouse Against Dcc Icd, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse against dcc icd/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    mouse against dcc icd - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    A Western blot of HEK293T cell lysates transfected with Dcc-pHluorin (Dcc-pH) and JamC-HALO. Immunoprecipitation was performed using GFP or negative control IgG antibodies. Blots were probed for Dcc and HALO-tag, with the yellow arrowhead indicating the expected JamC-HALO band size. B Schematic depicting interactions between Dcc, JamC, and the polarity protein Pard3. Pard3 recruits JamC to the membrane through its PDZ1 domain, which binds the Class 2 PDZ motif at JamC’s C-terminus. JamC interacts with Dcc’s extracellular domain, while Pard3’s PDZ3 the PDZ 3 domain is predicted to interact with a Class 1 PDZ binding motif (X-S/T-X-ϕ COOH ) , , present on Dcc intracellular domain. C , D Airyscan confocal imaging of CGNs nucleofected with Dcc-pHluorin (cyan), JamC-SNAP (yellow), and Halo-Pard3 (magenta). Phluorin and the SNAP dye used here are both pH sensitive highlighting membrane-bound proteins. C Single focal plane showing overlap of Dcc with JamC and Pard3 at the proximal dilation of a CGN (white arrowheads). D Maximum projection of two CGNs forming an adhesion, showing Dcc clustering at the adhesion site before and after Ntn1 addition (200 ng/L). Dcc co-localized with JamC/Pard3 at the adhesion (white arrowhead) and accumulated at the adhesion periphery (hollow arrowhead). Five minutes after the addition of Ntn1 at 200 ng/L, the number of bright Dcc clusters (blue arrowhead) at the membrane surface increased and some newly formed clusters were recruited to the periphery of the JamC/Pard3/Dcc-positive adhesion (white arrowhead). Proximity Labelling Assay (PLA) using Duolink™ fluorescence protocol on fixed dissociated granule neurons plated on laminin and cultured for 24 h, using 2 pairs of primary antibodies: Rabbit against Dcc extracellular domain (ECD) and a Goat against JamC ECD ( E ), and Mouse against Dcc intracellular domain (ICD) and a Rabbit against Pard3 ( F ). Duolink™ staining with no primary, only one or both primaries were compared. Bar graphs represent the ratio of PLA staining intensity (Gray) against Dapi (Cyan) intensity normalized to the negative control without primary antibody (E: n = 4, F: n = 4), replicated 4 (E) and 3 (F) times with similar results. Scale bars: (C, D) 5 µm, (E-F) 10 µm. Error bars represent SEM. See Source Data File.

    Journal: Nature Communications

    Article Title: Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit

    doi: 10.1038/s41467-024-55400-w

    Figure Lengend Snippet: A Western blot of HEK293T cell lysates transfected with Dcc-pHluorin (Dcc-pH) and JamC-HALO. Immunoprecipitation was performed using GFP or negative control IgG antibodies. Blots were probed for Dcc and HALO-tag, with the yellow arrowhead indicating the expected JamC-HALO band size. B Schematic depicting interactions between Dcc, JamC, and the polarity protein Pard3. Pard3 recruits JamC to the membrane through its PDZ1 domain, which binds the Class 2 PDZ motif at JamC’s C-terminus. JamC interacts with Dcc’s extracellular domain, while Pard3’s PDZ3 the PDZ 3 domain is predicted to interact with a Class 1 PDZ binding motif (X-S/T-X-ϕ COOH ) , , present on Dcc intracellular domain. C , D Airyscan confocal imaging of CGNs nucleofected with Dcc-pHluorin (cyan), JamC-SNAP (yellow), and Halo-Pard3 (magenta). Phluorin and the SNAP dye used here are both pH sensitive highlighting membrane-bound proteins. C Single focal plane showing overlap of Dcc with JamC and Pard3 at the proximal dilation of a CGN (white arrowheads). D Maximum projection of two CGNs forming an adhesion, showing Dcc clustering at the adhesion site before and after Ntn1 addition (200 ng/L). Dcc co-localized with JamC/Pard3 at the adhesion (white arrowhead) and accumulated at the adhesion periphery (hollow arrowhead). Five minutes after the addition of Ntn1 at 200 ng/L, the number of bright Dcc clusters (blue arrowhead) at the membrane surface increased and some newly formed clusters were recruited to the periphery of the JamC/Pard3/Dcc-positive adhesion (white arrowhead). Proximity Labelling Assay (PLA) using Duolink™ fluorescence protocol on fixed dissociated granule neurons plated on laminin and cultured for 24 h, using 2 pairs of primary antibodies: Rabbit against Dcc extracellular domain (ECD) and a Goat against JamC ECD ( E ), and Mouse against Dcc intracellular domain (ICD) and a Rabbit against Pard3 ( F ). Duolink™ staining with no primary, only one or both primaries were compared. Bar graphs represent the ratio of PLA staining intensity (Gray) against Dapi (Cyan) intensity normalized to the negative control without primary antibody (E: n = 4, F: n = 4), replicated 4 (E) and 3 (F) times with similar results. Scale bars: (C, D) 5 µm, (E-F) 10 µm. Error bars represent SEM. See Source Data File.

    Article Snippet: Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam 1:200 dil) and Goat against JamC (ECD) (AF1213, R&D systems, 1:50 dil), and Mouse against Dcc (ICD) (A-1, Santa Cruz 1:100 dil) and Rabbit against Pard3 (07-330, Sigma-Aldrich 1:200 dil).

    Techniques: Western Blot, Transfection, Immunoprecipitation, Negative Control, Membrane, Binding Assay, Imaging, Fluorescence, Cell Culture, Staining